Cy3 fluorescent spectra
WebOct 30, 2008 · We report on the role of dye–nucleobase interactions on the photophysical properties of the indocarbocyanine Cy3. The fluorescence efficiency and lifetime of Cy3 increase in the presence of all four nucleoside monophosphates. This behavior correlates with an increase in the activation energy for photoisomerization and a ∼4 nm red shift in … WebApr 13, 2024 · Over the past several years, resonance energy transfer involving noble metallic nanoparticles has received considerable attention. The aim of this review is to cover advances in resonance energy transfer, widely exploited in biological structures and dynamics. Due to the presence of surface plasmons, strong surface plasmon resonance …
Cy3 fluorescent spectra
Did you know?
WebThe BD Spectrum Viewer is a tool that depicts the excitation and emission curves of fluorochromes common to flow cytometry. This tool can be used to determine …
WebIn the UV–vis spectra , for the free IgG-Cy3, the absorbances at 280 nm and 552 nm were clearly observed, which were attributed to the protein and its labelled fluorescent molecules Cy3. For HmA@IgG-Cy3, the protein signal at 280 nm was not very remarkable, but it could still be observed. WebCy3 dye. Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids. The Cy3 fluorophore is also the basis for the lipophilic neuronal and long ...
WebJan 10, 2024 · With the help of fluorescent dyes, ... EpaxialMyf5 eGFP; immuno-stained for GFP-Alexa 488; embryonic muscle fibers stained with Desmin-Cy3, the nuclei are revealed with Hoechst Size from top to bottom: 3.5 mm (a), 800 µm (b). ... Good understanding of the spectra of the dyes used in the sample is also important to choose the right light source ... WebFluorescence SpectraViewer. Invitrogen Alexa Fluor 546 dye is a bright, orange-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. For stable signal generation in imaging and flow cytometry, …
WebCy3 dye. Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In …
WebThis is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength … honeybear brand applesWebDec 27, 2024 · Enzyme-activated fluorogenic probes, which invoke enzymatic catalysis to trigger the generation of fluorescence, provide a versatile platform for monitoring biological processes. The development of fluorogenic probes that can readily penetrate the cell envelopes of bacteria are essential to examine intracellular targets of live bacterial cells. … honey bear bottle walmartWebMay 6, 2015 · The hybridization was carried out using DNA targets labeled with a Cy3 fluorescent dye. The DNA target solution was diluted in a hybridization buffer solution (NaCl: 0.5 M, PBS: 0.01 M) and spread throughout the sample surface. ... The impedance spectra were analyzed with Z-fit within the EC-lab software (Bio-Logic, Claix, France) … honey bear breyer horseWebCy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC). Due to its high molar extinction coefficient, this … honeybear breyerWebTYE 563 can be used as a direct substitute for Cy3®. View product details for TYE™ 563: 5' 3' ... ATTO™ 647N exhibits strong absorption, high fluorescence quantum yield, and good pH and photo-stability. ATTO™ 647N can be used as a substitute for Alexa Fluor® 647. Dye calibration may be required for each commercial detection instrument. honeybear brandsWebCy3 and Cy5 are excited by the 488-nanometer line of an argon laser and the 633-nanometer line of a helium-neon diode or laser, respectively. These conjugates can be … honey bear bssWebAlexa Fluor 647 dye. Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high ... honey bear breakfast