2024 Lamp2b-ha

Lamp2b-ha

Author: dikt

August undefined, 2024

Tīmeklis2024. gada 1. dec. · The pcDNA GNSTM-3-Flag-10-Lamp2b-HA plasmid (Flag-Lamp2b) was obtained from AddGene (Cambridge, MA; AddGene plasmid #71293, originally provided by Joshua Leonard [21]). For construction of the ABP-Lamp2b fusion plasmid (pcDNA GNSTM-3-ABP-10-Lamp2b-HA), the cDNA fragment encoding … Tīmeklis2024. gada 24. dec. · Mutations in lysosomal-associated membrane protein 2 ( LAMP-2) gene are associated with Danon disease, which often leads to cardiomyopathy/heart failure through poorly defined mechanisms. Here, we identify the LAMP-2 isoform B (LAMP-2B) as required for autophagosome–lysosome fusion in human …

Mouse brain-wide transgene expression by systemic injection of ...

TīmeklisLamp2b-HA and T7-Lamp2b-HA contained the HA tag as fusion proteins of Lamp2b. GAPDH was detected as an internal control. CD63 was detected as an exosome … Tīmeklis2024. gada 20. jūl. · The construction of pRBP-Lamp2b-HA has been previously reported. 36 HA is a commonly used epitope tag, which does not interfere with the … oss 117 bande annonce 2021

pcDNA Flag Lamp2b-HA载体图谱质粒图谱、序列、价格、抗性、 …

Tīmeklis2024. gada 24. nov. · Lamp2b-HuR融合蛋白功能化的外泌体能够将被RNA结合基序识别的RNA靶标驱动至溶酶体以在受体细胞中降解。该系统在富含溶酶体的巨噬细胞中 … TīmeklispcDNA Flag Lamp2b-HA质粒价格1200元，pcDNA Flag Lamp2b-HA(爱迪基因#71292 )质粒图谱（map），质粒序列(Sequence)见下文，质粒抗性为氨苄青霉素，质粒大 … TīmeklisHuman Lamp2b cDNA clone 3543019 was purchased from Open Biosystems (now sold by Dharmacon) and inserted into pcDNA3.1+ Hygro (Clontech). A C-terminal GSG spacer and an HA peptide tag (YPYDVPDYA) were added by … oss 117 2021 streaming

Systemic AAV9.LAMP2B injection reverses metabolic and

Supplementary Figure 1. Western blot assay with anti-HA antibody …

Tīmeklis2015. gada 5. febr. · The Lamp2b fusion proteins were expressed at similar levels in cells, as indicated by the HA Western blot. However, the FLAG peptide on FLAG-Lamp2b-HA could not be detected, although the C-terminal FLAG peptide of Lamp2b-HA-FLAG was detected ( Fig. 1E ). The same pattern was observed in exosomes ( … TīmeklisDownload scientific diagram Identification of CBD-Lamp2b-HA encoding in the stable cell line. (A) The relative mRNA expression of fusion protein in negative control and … oss 117 actriceTīmeklis一种基于Lamp2b的融合蛋白可用于在表面显示狂犬病病毒糖蛋白（RVG），当这些胞外体被系统地给予时，表面展示的RVG导致这些胞外体在小鼠的神经元和大脑中积聚。去除Lamp2b RVG中的信号肽可显著降低RVG在工程化胞外体上的展示。其他用于融合蛋白外体表面显示的常用分子包括四跨膜蛋白（CD63、CD9、CD81）、糖基磷脂酰 … oss 117 film complet

"Tīmeklis2024. gada 9. apr. · Characterization of Genetically Engineered CAP-Lamp2b-Hemagglutinin (HA)-Exosomes and Lamp2b-Exosomes. We have developed a workflow to generate a novel brain targeted drug delivery system (DDS) that involves generation of genetically engineering exosomes by first selecting various functional AAV capsid … " - Lamp2b-ha

Lamp2b-ha

Tīmeklis2024. gada 4. jūn. · In this approach, exosome-producing cells were transiently transfected with a pcDNA GNSTM-3-RVG-10-Lamp2b-HA vector to express Lamp2b fusion protein fused in frame with the neuron-specific rabies ... Tīmeklis2024. gada 24. dec. · Mutations in lysosomal-associated membrane protein 2 ( LAMP-2) gene are associated with Danon disease, which often leads to cardiomyopathy/heart …

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TīmeklisGoat Anti Lamp2b, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … TīmeklisLamp2b ( LAMP2 Human) Use Tags HA Expression Mammalian Mutation Promoter CMV Availability Academic Institutions and Nonprofits only Enlarge pCAG-G-Flamp2 Plasmid #192782 Purpose green biosensor for cAMP in mammalian cells Depositor Jun Chu Article Liu et al Front Pharmacol. 2024 May 12;13:902290. Insert G-Flamp2 Use …

TīmeklisLOCUS pcDNA GNSTM-3-Flag-10-Lamp2b-HA 6925 bp ds-DNA circular SYN 06-AUG-2024 DEFINITION Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.. ACCESSION . VERSION . KEYWORDS pcDNA GNSTM-3-Flag-10 … TīmeklisSequences pcDNA GNSTM-3-RVG-10-Lamp2b-HA Sequences (3) Addgene Sequences: Partial (2) Depositing Scientist Sequences: Full (1) Full Sequences from …

TīmeklispcDNA GNSTM-3-Flag-10-Lamp2b-HA (Plasmid #71293) Print Enlarge View all sequences Purpose Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag. Depositing Lab Joshua Leonard Publication TīmeklispMEH60 Lamp2b MS2 HA (Plasmid #168209) Print Enlarge View all sequences Purpose Mammalian expression of lysosome-associated membrane protein 2b fused …

TīmeklisWestern blot analysis shows expression of fusion protein CAP-Lamp2b-HA in total protein isolated 36 hours after electroporation of CAP-Lamp2b with BM-MSCs as both the Hemagglutinin HA-tag antibody specific and Lamp2b-specific positive signal shows in the 1 st western blot run ( .

Tīmeklis2024. gada 9. apr. · GNSTM-3-FLAG10-CAP-Lamp2b-HA plasmid, the expression of CAP-Lamp2b-HA fusion protein, we assessed the protein level of Lamp2b and CAP-Lamp2b-HA fusion protein in BM-MSCs 48 hours after electroporation. Compared with BM-MSCs electroporated by pcDNA-Hygro-Lamp2bplasmid, the western blotting … oss 117 filmographieTīmeklisHA (C terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site NheI (destroyed during cloning) 3′ cloning site BamHI (destroyed during … oss 117 rio streaming english subtitleshttp://www.biofeng.com/adjy/zhili/pcDNA%20Flag%20Lamp2b-HA.html oss 117 rio ne répond plus streaming vf hdTīmeklis2015. gada 3. marts · Exosomes and cells were incubated for 2 h at 37 °C, as described (11). Cells were then washed with PBS and harvested for flow cytometry. Flow … oss 117 1 streaming vfTīmeklisCD51 + CD61 (Integrin alpha V + Integrin beta 3) CD51 / Integrin alpha V. CD52 oss 117 streaming freeTīmeklis860 Centennial Ave. • Piscataway, NJ 08854 USA • 1-732-885-9188, 1-877-436-7274 (Toll Free) • [email protected] oss 117 rio ne repond plus streaming vfLysosome-associated membrane protein 2 (LAMP2), also known as CD107b (Cluster of Differentiation 107b) and Mac-3, is a human gene. Its protein, LAMP2, is one of the lysosome-associated membrane glycoproteins. The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. … oss62852-f3