WebbProduct information. Hybridase Thermostable RNase H specifically degrades the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridized RNA. In contrast to E. coli RNase H, which is rapidly inactivated at 55°C, Hybridase RNase H is active at high temperatures. It has optimal activity above 65°C and can be used at temperatures up to 95°C. WebbA typical PCR thermocycling program consists of the following steps: 1. denature DNA (94°C) ~1 minute; 2. anneal primers to template (based on the melting temperature of …
WO2014131113A1 - Thermostable nuclease - Google Patents
Webb17 maj 2015 · This test is also known as the heat stable nuclease test. It is a 4hr test based on the production of a heat stable DNase (thermonuclease) by Staphylococcus aureus. It … Webb10 aug. 2011 · To achieve binding of the probe under conditions of the extension reaction with temperatures typically between 55°C and 70°C, modified bases such as locked nucleic acids (LNAs) or pendant groups such as a minor groove binder (MGB) are incorporated into the oligonucleotide to increase the T m [ 18 – 20 ]. permitted development change of use wales
Nuclease activity: an exploitable biomarker in bacterial infections
Webb1 jan. 2024 · NaME-PrO relies on the thermostable duplex-specific nuclease ... In combination with HRM, this approach yielded a detection limit of 0.01% ... Buhard O, Bui H, et al. Improved Microsatellite Instability Detection and Identification by Nuclease-Assisted Microsatellite Instability Enrichment Using HSP110 T17. Clin Chem 2024;64(8):1252 ... WebbDetermination of Staphylococcus aureus thermostable nuclease in foods (HC MFHPB-28) Top Determination of enterotoxin production by Staphylococcus aureus in foods and … WebbSubsequently, we conducted RNA detection assays over a range of temperatures from 37 to 72 °C to determine the optimal temperature for TccCas13a and HheCas13a. … permitted development and solar panels